Manual de Ecologia Viral Aquática - Livro Gratúito da ASLO

A American Society of Limnology and Oceanography, responsável pela publicação do jornal Limnology & Oceanography, publicou seu primeiro e-book online. O livro "Manual de Ecologia Viral Aquática" (MAVE, sig;a em inglês) foi editado por Steven Wilhelm, Weinbauer Markus e Suttle Curtis. O livro contém 19 capítulos refletindo o estado-da-arte e opiniões sobre diferentes abordagens para o estudo da ecologia viral em sistemas aquáticos. Os temas abordados no livro vão desde a contagem de vírus até técnicas moleculares para o estudo de populações e comunidades de vírus. O conteúdo deste e-book foi escolhido em consulta com o grupo de trabalho sobre vírus marinhos do Scientific Committee for Oceanographic Research, e a publicação apoiada pela Gordon and Betty Moore Foundation.

Para baixar o livro gratúito, clique no link abaixo:

Conteúdo do Livro
Markus G. Weinbauer, Janet M. Rowe, and Steven W. Wilhelm
Determining rates of virus production in aquatic systems by the virus reduction approach
Chapter 1, pp 1-8
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Ruth-Anne Sandaa, Steven M. Short, and Declan C. Schroeder
Fingerprinting aquatic virus communities
Chapter 2, pp 9-18
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André M. Comeau and Rachel T. Noble
Preparation and application of fluorescently labeled virus particles
Chapter 3, pp 19-29
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John H. Paul and Markus Weinbauer
Detection of lysogeny in marine environments
Chapter 4, pp 30-33
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Michael J. Allen, Bela Tiwari, Matthias E. Futschik, and Debbie Lindell
Construction of microarrays and their application to virus analysis
Chapter 5, pp 34-56
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Kenneth M. Stedman, Kate Porter, and Mike L. Dyall-Smith
The isolation of viruses infecting Archaea
Chapter 6, pp 57-64
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Susan A. Kimmance and Corina P. D. Brussaard
Estimation of viral-induced phytoplankton mortality using the modified dilution method
Chapter 7, pp 65-73
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Roberto Danovaro and Mathias Middelboe
Separation of free virus particles from sediments in aquatic systems
Chapter 8, pp 74-81
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Steven M. Short, Feng Chen, and Steven W. Wilhelm
The construction and analysis of marker gene libraries
Chapter 9, pp 82-91
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Keizo Nagasaki and Gunnar Bratbak
Isolation of viruses infecting photosynthetic and nonphotosynthetic protists
Chapter 10, pp 92-101
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Corina P.D. Brussaard, Jérôme P. Payet, Christian Winter, and Markus G. Weinbauer
Quantification of aquatic viruses by flow cytometry
Chapter 11, pp 102-109
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K. Eric Wommack, Télesphore Sime-Ngando, Danielle M. Winget, Sanchita Jamindar, and Rebekah R. Helton
Filtration-based methods for the collection of viral concentrates from large water samples
Chapter 12, pp 110-117
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Mathias Middelboe, Amy M. Chan, and Sif K. Bertelsen
Isolation and life cycle characterization of lytic viruses infecting heterotrophic bacteria and cyanobacteria
Chapter 13, pp 118-133
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William H. Wilson and Declan Schroeder
Sequencing and characterization of virus genomes
Chapter 14, pp 134-144
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Curtis A. Suttle and Jed A. Fuhrman
Enumeration of virus particles in aquatic or sediment samples by epifluorescence microscopy
Chapter 15, pp 145-153
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Grieg F. Steward and Alexander I. Culley
Extraction and purification of nucleic acids from viruses
Chapter 16, pp 154-165
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Janice E. Lawrence and Grieg F. Steward
Purification of viruses by centrifugation
Chapter 17, pp 166-181
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Hans-W. Ackermann and Mikal Heldal
Basic electron microscopy of aquatic viruses
Chapter 18, pp 182-192
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Alexander I. Culley, Curtis A. Suttle, and Grieg F. Steward
Characterization of the diversity of marine RNA viruses
Chapter 19, pp 193-201
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